cellTIRF

Total internal reflection fluorescence microscopy (TIRF) makes use of specific optics to produce illumination light only at the 50-100 nm range at the interface of the slide, massively reducing out of focus light and improving the ability to detect fluorescent molecules. Because of its low light intensity and high spatial resolution, it is a key technique in live-cell imaging. Designed for membrane dynamics, single molecule detection, and colocalization experiments, the IXplore TIRF microscope system offers simultaneous multicolor TIRF imaging for up to 4 colors with high stability. The Total Internal Reflection Fluorescence (TIRF) technique is the ideal method for observations of cells close to the coverslip surface.