stochastic optical reconstruction microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM) exploits the photoswitching properties of some fluorophores. In certain conditions, fluorophores can be sent to an intermediary state (the “dark state”) from which they randomly cycle between ON and OFF states.
In the proper chemical and excitation conditions the transition to the dark state is in the range of milliseconds to minutes and, importantly, is a completely stochastic event. As a result, when in the right chemical and excitation conditions, fluorophores start blinking randomly and in an asynchronous manner

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